Abstract: This paper builds a theoretical framework by field survey of land-lost peasants’ entrepreneurship. The key factors of land-lost peasants’ entrepreneurship intention by using Logistic regression analysis method. The logic structural relationships between various factors are mined by using ISM model, and the factors chain with interrelated and clear hierarchy is built in order to clarify the internal mechanism of land-lost peasants’ entrepreneurship intention. Moreover, the policy recommendations of guiding land-lost peasants’ entrepreneurship are proposed in this paper. The results show that the entrepreneurship intention of land-lost peasants is significantly influenced by five factors which include gender, achievement motivation, innovation-oriented，expropriated bit and entrepreneurial behavior. These factors are at different levels and are interdependent and interrelated. Specifically, entrepreneurial behavior is a direct surface factors, innovation-oriented is an indirect intermediate layer factors, and gender, achievement motivation and expropriated bit are the deep roots factors. The results could provide a reference for solving the land-lost peasants’ employment and sustainable livelihoods.