Abstract: Taking the enterprise in different regions as targets, work-family conflict as mediate variable, and organizational support as moderate variable, this paper explores the impact of employee assistance programs on the employees’ turnover intention. 235 employees of diffent enterprise in Jiangsu, Anhui and Shanghai Province were tested through a questionnaire.The results show: EAP plays a negative role on turnover intention; work-family conflict plays a mediating role between employee assistance programs and turnover intention; EAP plays a negative role on work-family conflict, in which perceived organizational support can also play a role of mediated-moderator. Based on the results this paper lists three suggestions including: build and deploy EAP system, implement employee role conflict management and create a working environment for supporting the organization.