Abstract: E-commerce is an important attempt to change the traditional distribution channels for the agricultural industry, but also a new trend of agricultural materials market diversifying and technical service informationzing in the future. Due to the particularity of agricultural materials products and purchasing subjects, currently agricultural materials’ electronic suppliers adopt the operating method of online transactions drived offline service. From the trust transfer’s perspective, we empirically researched and compared the different impact of farmer’s offline trust on online trust and the different role of online and offline perception regulating the relationship of offline trust and online trust. The research shows that: offline trust had a positive effect on online trust, offline brand trust had the most impact on online ability-based trust, closely followed by online integrity-based trust, online benevolence-based trust were weakest, but offline retailers trust had the most impact on online benevolence-based trust, closely followed by online integrity-based trust, had no impact on online ability -based trust; of the relationship between offline brand trust and online trust, the regulating effect of perceived differentiation is the strongest, closely followed by perceived integration, perceived consistency is weakest, but of the relationship between offline retailers trust and online trust, the regulating effect of perceived consistency is the strongest, closely followed by perceived integration and perceived differentiation had no regulating effect.